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215、在限制性位点序列中，n意指核苷酸可为a、c、g或t；b意指核苷酸可为c、g或t；y意指核苷酸可为c或t；w意指核苷酸可为a或t；r意指核苷酸可为a或g；以及d意指a、g或t。
By “in trans” is meant herein Having said that protein, peptid, RNA, nucleic acid, molecule or any combination thereof 閱讀完整文章 isn't encoded on precisely the same nucleic acid molecule since the 1 comprising the origin of replication. In a specific embodiment, stated protein, peptid, RNA, nucleic acid, molecule or any combination thereof is encoded on the chromosome or on the plasmid.
in a few embodiments, the vector may possibly comprise a terminator sequence, or terminator. A “terminator,” as used herein, is actually a nucleic acid sequence that causes transcription to halt. A terminator may be unidirectional or bidirectional. it is actually comprised of a DNA sequence involved with particular termination of the RNA transcript by an RNA polymerase.
By “ecosystem” is supposed herein all the elements which surround a species and amid which some instantly or indirectly add to your subsistence of reported species.
fifteen. the strategy In accordance with embodiment fourteen, whereby explained conditional origin of replication is active in said donor bacterial mobile mainly because said donor bacterial mobile expresses reported provided protein, peptid, nucleic acid, RNA, molecule or any mix thereof.
To achieve this, the inventors analyzed the 282-bp PICI origin and found that it contains the O157 restriction internet site GAAABCC (GAAAGCC). The inventors modified This website within the origin and acquired the sequence GAAAGCa (smaller cap represents the mutation introduced) which shouldn't be identified by O157 strains.
The procedure must be adequately unusual in prospective focus on strains regarding reduce the pitfalls of spread and recombination,
The current creation more considerations the next embodiments. one. A method for in vivo modulating the microbiome of a number organism by providing a nucleic acid of fascination right into a specific receiver bacterial mobile of reported microbiome, reported nucleic acid of curiosity making a specified impact on claimed qualified receiver bacterial cell, whereby mentioned system comprises administering, in explained organism, a nucleic acid vector comprising explained nucleic acid of interest, whereby explained vector even more comprises a conditional origin of replication that is inactive inside the qualified receiver bacterial cell but is Energetic in a very donor bacterial mobile, and mentioned vector is devoid of antibiotic resistance marker,
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A non-exhaustive listing of bacterial genera as well as their recognised host-specific micro organism viruses is introduced in the subsequent paragraphs.
260、细菌噬菌体可选自肌尾噬菌体科(非限制性地比如以下属：cp220病毒、cp8病毒、ea214病毒、felixo1病毒、moogle病毒、susp病毒、hp1病毒、p2病毒、kay病毒、p100病毒、silvia病毒、spo1病毒、tsarbomba病毒、twort病毒、cc31病毒、jd18病毒、js98病毒、kp15病毒、moon病毒、rb49病毒、rb69病毒、s16病毒、schizot4病毒、sp18病毒、t4病毒、cr3病毒、se1病毒、v5病毒、abouo病毒、agate病毒、agrican357病毒、ap22病毒、arv1病毒、b4病毒、bastille病毒、bc431病毒、bcep78病毒、bcepmu病毒、biquarta病毒、bxz1病毒、cd119病毒、cp51病毒、cvm10病毒、eah2病毒、el病毒、hapuna病毒、jimmer病毒、kpp10病毒、m12病毒、machina病毒、martha病毒、msw3病毒、mu病毒、myohalo病毒、nit1病毒、p1病毒、pakpuna病毒、pbuna病毒、phikz病毒、rheph4病毒、rsl2病毒、rsluna病毒、secunda5病毒、sep1病毒、spn3病毒、svuna病毒、tg1病毒、vhml病毒和wph病毒)。
distinct promoters with various strengths could possibly be Employed in the existing invention bringing about unique amounts of gene/protein expression (e.g. the extent of expression initiated from an mRNA originating from the weak promoter is decreased than the level of expression initiated from a powerful promoter).